The droplet size was not strongly affected by the storage time and no visible precipitation was observed. We have demonstrated in this study that the optimized AG-NE provided significantly enhanced bioavailability of AG compared with that provided by the AG suspension administered through the same route. Abbreviation: PO, oral administration. The enhanced oral bioavailability can be attributed to the enhancement of AG solubility and intestinal permeability and the reduction of the droplet size in the formulation. Selectivity was confirmed by the absence of interference peaks at the retention time of AG in chromatograms of blank rat plasma.
Nanoemulsion NE is a promising technology for enhancing the oral bioavailability of poorly soluble drugs. These results demonstrate that the AG-NE is effective for improving the oral bioavailability of AG, and thus exhibits great potential for future clinical application as an IBD treatment strategy. These results demonstrate that the AG-NE is effective for improving the oral bioavailability of AG, and thus exhibits great potential for future clinical application as an IBD treatment strategy. The mobile phase consisted of acetonitrile and 0.
The extraction recoveries for AG were According to a previous study, ethanol likely enhances quercetin absorption depending on the ethanol concentration because of the enhancement of quercetin solubility. An appropriate combination of surfactants with low and high HLB values leads to the formation of a stable NE upon dilution with water.
An appropriate combination of surfactants with low and high HLB values leads to the formation of a stable NE upon dilution with water. Blood samples were collected as blank plasma before drug administration and at predetermined time points after administration. The plasma concentration of AG was determined using our validated high performance liquid chromatography method, which was used to generate a linear calibration curve over the concentration range of 0. Pharmacokinetic study Male Sprague—Dawley rats were anesthetized, and polyethylene tubes were implanted into the right jugular and femoral veins for repeated sampling and intravenous injection. Pharmacokinetic parameters were calculated using non-compartmental analysis.
In this study, the mean droplet size of AG-NE was approximately nm and the low solubility of AG improved, as evidenced by the solubility test results. High surfactant concentrations in the SMEDD formulation might irritate the gastrointestinal tract, and the proposed solid lipid nanoparticle formulation lacked stability data to ensure its feasibility.
The extraction recoveries for AG were The intra-day accuracy of the results for AG in plasma was Among the mixtures of oils and ethanol, the solubility values of AG in triacetin The high-energy approach can be achieved through ultrasonicators, microfluidizers, and high-pressure homogenizers. A previous study showed that pretreatment with the ethanolic extract of A. Pharmacokinetic studies show that compared with the AG suspension, the optimized AG-NE increased the relative bioavailability of AG approximately 6-fold.
The mobile phase consisted of acetonitrile and 0.
We also evaluated the pharmacokinetics of AG after oral administration of optimized AG-NE to rats and compared its bioavailability with that of an AG suspension. Among all the formulations, F1 formed a semi-solid state after high-speed homogenization for 10 min. Subsequently, the stock solution was diluted with the mobile phase to prepare the working solutions at concentrations ranging from 0.